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<DIV><FONT face=Arial size=2>ale jie vystosi labai sparciai su savo tom
vitrification, per 20</FONT></DIV>
<DIV><FONT face=Arial size=2>metu tipo labai dideli progresa padare, yra
ten kazkur mikroskopines</FONT></DIV>
<DIV><FONT face=Arial size=2>fotkes atsildytu smegeneliu, tipo nesimato jokio
damage :)</FONT></DIV>
<DIV><FONT face=Arial size=2>Speju po kokiu 40 metu dar kelis kart
patobules.</FONT></DIV>
<DIV><FONT face=Arial size=2>Tik baisiausia, jei po poros simtu metu prikeltu
kazkokiu tai daunu :(</FONT></DIV>
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style="PADDING-RIGHT: 0px; PADDING-LEFT: 5px; MARGIN-LEFT: 5px; BORDER-LEFT: #000000 2px solid; MARGIN-RIGHT: 0px">
<DIV>"flegmatoid" <<A
href="mailto:flegmatoid@gmail.com">flegmatoid@gmail.com</A>> wrote in
message <A
href="news:hjudke$2mc$1@trimpas.omnitel.net">news:hjudke$2mc$1@trimpas.omnitel.net</A>...</DIV>
<DIV><FONT face=Arial size=2>pasiskaitinejus "FAQ for Scientist" skyreli,
pernelyg optimistiskai nenuteikia:</FONT></DIV>
<DIV><FONT face=Arial size=2></FONT><FONT face=Arial
size=2></FONT> </DIV>
<DIV><FONT face=Arial size=2>"Although much interest has attended the
cryopreservation of immature neurons for subsequent therapeutic intracerebral
transplantation, there are no reports on the cryopreservation of organized
adult cerebral tissue slices of potential interest for pharmaceutical drug
development. We report here the first experiments on cryopreservation of
mature rat transverse hippocampal slices. Freezing at 1.2 degrees C/min to -20
degrees C or below using 10 or 30% v/v glycerol or 20% v/v dimethyl sulfoxide
yielded extremely poor results. Hippocampal slices were also rapidly
inactivated by simple exposure to a temperature of 0 degree C in artificial
cerebrospinal fluid (aCSF). This effect was mitigated somewhat by 0.8 mM
vitamin C, the use of a more "intracellular" version of aCSF having reduced
sodium and calcium levels and higher potassium levels, and the presence of a
25% w/v mixture of dimethyl sulfoxide, formamide, and ethylene glycol ("V(EG)
solutes"; Cryobiology 48, pp. 22-35, 2004). It was not mitigated by glycerol,
aspirin, indomethacin, or mannitol addition to aCSF. When RPS-2 (Cryobiology
21, pp. 260-273, 1984) was used as a carrier solution for up to 50% w/v V(EG)
solutes, 0 degree C was more protective than 10 degrees C. Raising V(EG)
concentration to 53% w/v allowed slice vitrification without injury from
vitrification and rewarming per se, but was much more damaging than exposure
to 50% w/v V(EG). <STRONG>This problem was overcome by using the analogous 61%
w/v VM3 vitrification solution (Cryobiology 48, pp. 157-178, 2004) containing
polyvinylpyrrolidone and two extracellular "ice blockers." With VM3, it was
possible to attain a tissue K(+)/Na(+) ratio after vitrification ranging from
91 to 108% of that obtained with untreated control slices. Microscopic
examination showed severe damage in frozen-thawed slices, but generally good
to excellent ultrastructural and histological preservation after
vitrification.</STRONG> Our results provide the first demonstration that both
the viability and the structure of mature organized, complex neural networks
can be well preserved by vitrification. These results may assist
neuropsychiatric drug evaluation and development and the transplantation of
integrated brain regions to correct brain disease or injury."</FONT></DIV>
<DIV><FONT face=Arial size=2></FONT><FONT face=Arial
size=2></FONT> </DIV>
<DIV><FONT face=Arial size=2>reziume - uzsaldyt iseina su nedideliais
nuostoliais(tipo), bet atsilus nieko nelieka.</FONT></DIV>
<DIV><FONT face=Arial size=2></FONT> </DIV>
<DIV><FONT face=Arial size=2>"bzik" <</FONT><A
href="mailto:bzik@bzik.bzik"><FONT face=Arial
size=2>bzik@bzik.bzik</FONT></A><FONT face=Arial size=2>> wrote in message
</FONT><A href="news:hjphf2$aqc$1@trimpas.omnitel.net"><FONT face=Arial
size=2>news:hjphf2$aqc$1@trimpas.omnitel.net</FONT></A><FONT face=Arial
size=2>...</FONT></DIV><FONT face=Arial size=2>> sakyciau brangiai
parduodama iliuzija. Jei jie atgaivintu gyvenimui pries koki desimtmeti
uzsaldyta kuneli, gal butu itikinamiau. <BR>> asmeniskai nei amzinam
gyvenimui, nei atsigaivinimui po simto metu nematau prasmes :) isivaizduok 19a
60-meti siandieninej aplinkoj. Gal prie kazko sugebetu adaptuotis, bet visumoj
liktu antrarusiu padaru aplinkiniu pajuokai. O gal po pirmo pasimaitinimo
desra su E kojas pakratytu :)<BR>> <BR>> "Necris" <</FONT><A
href="mailto:abc@def.aef"><FONT face=Arial size=2>abc@def.aef</FONT></A><FONT
face=Arial size=2>> wrote in message </FONT><A
href="news:hjpg23$899$1@trimpas.omnitel.net"><FONT face=Arial
size=2>news:hjpg23$899$1@trimpas.omnitel.net</FONT></A><FONT face=Arial
size=2>...<BR>>> $80.000 uz galva, $150.000 uz visa kuna. Ka
manot?:)<BR>>> MKN, ideja pakankamai moksliskai pagrista ir
isvystyta,<BR>>> kad butu galima tiketis kazkokiu rezultatu (jei
nebankrutuos<BR>>> po poros simtu metu:)<BR>></FONT>
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