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<DIV>"The risk of inadequate preservation may be ruled out completely within
another decade if techniques for transplantable organ banking can be adapted to
achieve reversible preservation of the brain".</DIV>
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<DIV>"Necris" <<A href="mailto:abc@def.aef">abc@def.aef</A>> wrote in
message <A
href="news:hjugtg$7v1$1@trimpas.omnitel.net">news:hjugtg$7v1$1@trimpas.omnitel.net</A>...</DIV>
<DIV><FONT face=Arial size=2>ale jie vystosi labai sparciai su savo tom
vitrification, per 20</FONT></DIV>
<DIV><FONT face=Arial size=2>metu tipo labai dideli progresa padare, yra
ten kazkur mikroskopines</FONT></DIV>
<DIV><FONT face=Arial size=2>fotkes atsildytu smegeneliu, tipo nesimato jokio
damage :)</FONT></DIV>
<DIV><FONT face=Arial size=2>Speju po kokiu 40 metu dar kelis kart
patobules.</FONT></DIV>
<DIV><FONT face=Arial size=2>Tik baisiausia, jei po poros simtu metu prikeltu
kazkokiu tai daunu :(</FONT></DIV>
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<DIV>"flegmatoid" <<A
href="mailto:flegmatoid@gmail.com">flegmatoid@gmail.com</A>> wrote in
message <A
href="news:hjudke$2mc$1@trimpas.omnitel.net">news:hjudke$2mc$1@trimpas.omnitel.net</A>...</DIV>
<DIV><FONT face=Arial size=2>pasiskaitinejus "FAQ for Scientist" skyreli,
pernelyg optimistiskai nenuteikia:</FONT></DIV>
<DIV><FONT face=Arial size=2></FONT><FONT face=Arial
size=2></FONT> </DIV>
<DIV><FONT face=Arial size=2>"Although much interest has attended the
cryopreservation of immature neurons for subsequent therapeutic
intracerebral transplantation, there are no reports on the cryopreservation
of organized adult cerebral tissue slices of potential interest for
pharmaceutical drug development. We report here the first experiments on
cryopreservation of mature rat transverse hippocampal slices. Freezing at
1.2 degrees C/min to -20 degrees C or below using 10 or 30% v/v glycerol or
20% v/v dimethyl sulfoxide yielded extremely poor results. Hippocampal
slices were also rapidly inactivated by simple exposure to a temperature of
0 degree C in artificial cerebrospinal fluid (aCSF). This effect was
mitigated somewhat by 0.8 mM vitamin C, the use of a more "intracellular"
version of aCSF having reduced sodium and calcium levels and higher
potassium levels, and the presence of a 25% w/v mixture of dimethyl
sulfoxide, formamide, and ethylene glycol ("V(EG) solutes"; Cryobiology 48,
pp. 22-35, 2004). It was not mitigated by glycerol, aspirin, indomethacin,
or mannitol addition to aCSF. When RPS-2 (Cryobiology 21, pp. 260-273, 1984)
was used as a carrier solution for up to 50% w/v V(EG) solutes, 0 degree C
was more protective than 10 degrees C. Raising V(EG) concentration to 53%
w/v allowed slice vitrification without injury from vitrification and
rewarming per se, but was much more damaging than exposure to 50% w/v V(EG).
<STRONG>This problem was overcome by using the analogous 61% w/v VM3
vitrification solution (Cryobiology 48, pp. 157-178, 2004) containing
polyvinylpyrrolidone and two extracellular "ice blockers." With VM3, it was
possible to attain a tissue K(+)/Na(+) ratio after vitrification ranging
from 91 to 108% of that obtained with untreated control slices. Microscopic
examination showed severe damage in frozen-thawed slices, but generally good
to excellent ultrastructural and histological preservation after
vitrification.</STRONG> Our results provide the first demonstration that
both the viability and the structure of mature organized, complex neural
networks can be well preserved by vitrification. These results may assist
neuropsychiatric drug evaluation and development and the transplantation of
integrated brain regions to correct brain disease or injury."</FONT></DIV>
<DIV><FONT face=Arial size=2></FONT><FONT face=Arial
size=2></FONT> </DIV>
<DIV><FONT face=Arial size=2>reziume - uzsaldyt iseina su nedideliais
nuostoliais(tipo), bet atsilus nieko nelieka.</FONT></DIV>
<DIV><FONT face=Arial size=2></FONT> </DIV>
<DIV><FONT face=Arial size=2>"bzik" <</FONT><A
href="mailto:bzik@bzik.bzik"><FONT face=Arial
size=2>bzik@bzik.bzik</FONT></A><FONT face=Arial size=2>> wrote in
message </FONT><A href="news:hjphf2$aqc$1@trimpas.omnitel.net"><FONT
face=Arial size=2>news:hjphf2$aqc$1@trimpas.omnitel.net</FONT></A><FONT
face=Arial size=2>...</FONT></DIV><FONT face=Arial size=2>> sakyciau
brangiai parduodama iliuzija. Jei jie atgaivintu gyvenimui pries koki
desimtmeti uzsaldyta kuneli, gal butu itikinamiau. <BR>> asmeniskai nei
amzinam gyvenimui, nei atsigaivinimui po simto metu nematau prasmes :)
isivaizduok 19a 60-meti siandieninej aplinkoj. Gal prie kazko sugebetu
adaptuotis, bet visumoj liktu antrarusiu padaru aplinkiniu pajuokai. O gal
po pirmo pasimaitinimo desra su E kojas pakratytu :)<BR>> <BR>>
"Necris" <</FONT><A href="mailto:abc@def.aef"><FONT face=Arial
size=2>abc@def.aef</FONT></A><FONT face=Arial size=2>> wrote in message
</FONT><A href="news:hjpg23$899$1@trimpas.omnitel.net"><FONT face=Arial
size=2>news:hjpg23$899$1@trimpas.omnitel.net</FONT></A><FONT face=Arial
size=2>...<BR>>> $80.000 uz galva, $150.000 uz visa kuna. Ka
manot?:)<BR>>> MKN, ideja pakankamai moksliskai pagrista ir
isvystyta,<BR>>> kad butu galima tiketis kazkokiu rezultatu (jei
nebankrutuos<BR>>> po poros simtu metu:)<BR>></FONT>
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